New Project on Molecular Cytogenetics and Genomics of Ensete banana

We have a research project starting in 2017 on Ethiopian banana, Ensete ventricosum, for 18 months, in collaboration with Royal Botanic Gardens, Kew (Dr Paul Wilkin) and University of Addis Ababa (Professor Sebsebe Demissew). There is a post-doctoral position working with the project (closed 7 August 2017). The project “Modelling and genomics resources to enhance…

The landscape and structural diversity of LTR Retrotransposons in the Musa genome

334. Nouroz F, Noreen S, Ahmad H, Heslop Harrison JS(P). 2017. The landscape and structural diversity of LTR Retrotransposons in the Musa genome. Molecular Genetics and Genomics 292: 1051-1067. http://dx.doi.org/10.1007/s00438-017-1333-1 Publisher site (££$$€€ needs subscription) Author version of manuscript: Nouroz_MGG2017_MusaLTRretrotransposons_AuthorVer Long terminal repeat retrotransposons represent a major component of plant genomes and act as drivers of genome…

Analysis of Petunia vein clearing virus (PVCV) sequences, retroelements and tandem repeats in Petunia axillaris N and P. inflata S6

325a. Schwarzacher T, Heslop-Harrison JS, Richert-Pöggeler KR. 2016. Analysis of Petunia vein clearing virus (PVCV) sequences, retroelements and tandem repeats in Petunia axillaris N and P. inflata S6. Supplementary manuscript 2. Nature Plants 2: article number 16074. Link to manuscript: Petunia_PVCV_Repeats_SchwarzacherEtAl2016 Within the genome sequence assemblies of P. axillaris (PaxiN) and P. inflata (PinfS6) and unassembled reads, we…

Animal Cytogenetics, Gene Mapping and Chromosome Research – The 21st Colloquium Ischia, Naples, Italy

Cytogenetics of animals is moving forward rapidly with the integration of sequencing and robust phylogenies with methods of chromosome analysis. The 21st International Colloquium on Animal Cytogenetics and Gene Mapping held June 2014 covered a range of topics in comparative, molecular, veterinary and environmental cytogenetics, as well as a session on cytogenetics of non-mammalian vertebrates and…

In situ hybridization: a course on FISH and ChIp

In situ hybridization – a course on FISH and ChIp In situ hybridization, now largely with fluorescent detection so ‘fluorescent in situ hybridization’ or FISH, is a key method for localizing DNA sequences along chromosomes and occassionally within interphase nuclei. RNA in situ hybridization (not discussed here) is used for locating gene transcripts within nuclei….

Haploid callus from a diploid Musa accession for genome analysis

303. Nair AS, Heslop-Harrison P, Schwarzacher T. 2013. Production of haploid tissues and SNP analysis of the genome in Musa acuminata cv.‘Matti’ (AA). Plant Mutation Reports 3(1): 18-24.   Haploid and doubled haploid plants are of considerable value in genetic studies, genomics and plant breeding, allowing characterization and exploitation of genes where only one allele is present. Inbred banana…