Publication list from Molecular Cytogenetics and Plant Genome Evolution Groups References J.S. Pat Heslop-Harrison, Trude Schwarzacher
Category: Techniques
IAEA-FAO Manual on mutation breeding and introduction to plant breeding and selection
346. Nielen S., Forster BP, Heslop-Harrison JS. 2018. Mutagen effects in the first generation after seed treatment: biological effects of mutation treatments. Chapter 4 In: FAO/IAEA. Manual on Mutation Breeding – Third edition. Spencer-Lopes MM, Forster BP, Jankuloski L (eds), Food and Agriculture Organization of the United Nations. Rome, Italy. 301 pp. Free download of whole book:…
ImmunoFISH – Simultaneous visualisation of proteins and DNA sequences gives insight into meiotic processes in nuclei of grasses
Sepsi A, Fábián A, Jäger K, Heslop-Harrison JS, Schwarzacher T. ImmunoFISH: simultaneous visualisation of proteins and DNA sequences gives insight into meiotic processes in nuclei of grasses. Frontiers in Plant Science. 9: 1193. https://doi.org/10.3389/fpls.2018.01193 ImmunoFISH is a method combining immunolabelling (IL) with fluorescent in situ hybridisation (FISH) to simultaneously detect the nuclear distribution of proteins and specific DNA…
Preparation and fluorescent analysis of plant metaphase chromosomes
TS. Schwarzacher T. 2016. Preparation and fluorescent analysis of plant metaphase chromosomes. Chapter 7. In: Plant Cell Division: Methods and Protocols, ed Caillaud M-C; Methods in Molecular Biology 1370: 87-103. doi: 10.1007/978-1-4939-3142-2_7. Humana Press, Springer, New York. Local Copy: Chromosome Preparation. Trude Schwarzacher. http://www.molcyt.com Good preparations are essential for informative analysis of both somatic and…
Chromosomal distribution and evolution of retrotransposons in diploid and polyploid Brachiaria forage grasses
319. Santos FC, Guyot R, Valle CB do, Chiari L, Techio VH, Heslop-Harrison P, Vanzela ALL. 2015. Chromosomal distribution and evolution of abundant retrotransposons in plants: gypsy elements in diploid and polyploid Brachiaria forage grasses. Chromosome Research 23(3): 571-582. DOI: 10.1007/s10577-015-9492-6 Text Brachiaria_Fabiola_Santos_etal AuthorVersion. Abstract: Like other eukaryotes, the nuclear genome of plants consists of DNA with…
In situ hybridization: a course on FISH and ChIp
In situ hybridization – a course on FISH and ChIp In situ hybridization, now largely with fluorescent detection so ‘fluorescent in situ hybridization’ or FISH, is a key method for localizing DNA sequences along chromosomes and occassionally within interphase nuclei. RNA in situ hybridization (not discussed here) is used for locating gene transcripts within nuclei….
Spectroscopy and DNA concentration measurement
DNA concentration measurement is fundamental to most molecular biology methods. What is the basis of DNA concentration measurement? I’m always happy to find an article which has detail at the level you need to know! In a free magazine, VWR BioMarkers, there is a very useful article from GE Life Sciences. http://media.vwr.com/interactive/publications/VWR_bioMarke_2013/Magazine/files/assets/basic-html/index.html#7 A local copy…