Category Archives: Techniques

Preparation and fluorescent analysis of plant metaphase chromosomes

TS. Schwarzacher T. 2016. Preparation and fluorescent analysis of plant metaphase chromosomes. Chapter 7. In: Plant Cell Division: Methods and Protocols, ed Caillaud M-C; Methods in Molecular Biology 1370: 87-103. doi: 10.1007/978-1-4939-3142-2_7. Humana Press, Springer, New York. Local Copy: Chromosome … Continue reading

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Chromosomal distribution and evolution of retrotransposons in diploid and polyploid Brachiaria forage grasses

319. Santos FC, Guyot R, Valle CB do, Chiari L, Techio VH, Heslop-Harrison P, Vanzela ALL. 2015. Chromosomal distribution and evolution of abundant retrotransposons in plants: gypsy elements in diploid and polyploid Brachiaria forage grasses. Chromosome Research 23(3): 571-582. DOI: 10.1007/s10577-015-9492-6 Text … Continue reading

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In situ hybridization: a course on FISH and ChIp

In situ hybridization – a course on FISH and ChIp In situ hybridization, now largely with fluorescent detection so ‘fluorescent in situ hybridization’ or FISH, is a key method for localizing DNA sequences along chromosomes and occassionally within interphase nuclei. … Continue reading

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Spectroscopy and DNA concentration measurement

DNA concentration measurement is fundamental to most molecular biology methods. What is the basis of DNA concentration measurement? I’m always happy to find an article which has detail at the level you need to know! In a free magazine, VWR … Continue reading

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