Sarbast Ihsan Mustafa, Trude Schwarzacher and John S. Heslop-Harrison The Nature and Chromosomal Landscape of Endogenous Retroviruses (ERVs) Integrated in the Sheep Nuclear Genome. DNA 2022, 2(1), 86-103; Free full text: https://doi.org/10.3390/dna2010007 Endogenous retroviruses (ERVs) represent genomic components of retroviral origin that are found integrated in the genomes of various species of vertebrates. These genomic…
Tag: in situ hybridization
Complete mitogenomes from Kurdistani sheep – abundant centromeric nuclear copies representing diverse ancestors
341. Mustafa SI, Schwarzacher T, and Heslop-Harrison JS. 2018. Complete mitogenomes from Kurdistani sheep: abundant centromeric nuclear copies representing diverse ancestors. Mitochondrial DNA Part A https://doi.org/10.1080/24701394.2018.1431226 (publisher – see free publisher link below if you can’t access) AND Mustafa et al_2018 AuthorVersion The geographical center of domestication and species diversity for sheep (Ovis aries) lies around the…
CENH3 morphogenesis reveals dynamic centromere associations during synaptonemal complex formation and the progression through male meiosis in hexaploid wheat
330. Sepsi A, Higgins JD, Heslop-Harrison JS, Schwarzacher T. 2017. CENH3 morphogenesis reveals dynamic centromere associations during synaptonemal complex formation and the progression through male meiosis in hexaploid wheat. Plant Journal 89: 235-249. http://dx.doi.org/10.1111/tpj.13379 and Author Version: Sepsi et al. 2016. CENH3 and wheat meiosis. Plant Journal. See bottom of page for YouTube video links. During…
Introgression of chromosome segments from multiple alien species in wheat breeding lines with wheat streak mosaic virus resistance
324. Ali N, Heslop-Harrison JS, Ahmad H, Graybosch RA, Hein GL, Schwarzacher T. 2016. Introgression of chromosome segments from multiple alien species in wheat breeding lines with wheat streak mosaic virus resistance. Heredity (2016) 117, 114–123; published online 1 June 2016 doi:10.1038/hdy.2016.36 Author version: N_Ali_et al 2016 Multiple Alien Introgressions in Wheat Publisher site: http://www.nature.com/hdy/journal/vaop/ncurrent/pdf/hdy201636a.pdf Pyramiding of…
Analysis of Petunia vein clearing virus (PVCV) sequences, retroelements and tandem repeats in Petunia axillaris N and P. inflata S6
325a. Schwarzacher T, Heslop-Harrison JS, Richert-Pöggeler KR. 2016. Analysis of Petunia vein clearing virus (PVCV) sequences, retroelements and tandem repeats in Petunia axillaris N and P. inflata S6. Supplementary manuscript 2. Nature Plants 2: article number 16074. Link to manuscript: Petunia_PVCV_Repeats_SchwarzacherEtAl2016 Within the genome sequence assemblies of P. axillaris (PaxiN) and P. inflata (PinfS6) and unassembled reads, we…
Preparation and fluorescent analysis of plant metaphase chromosomes
TS. Schwarzacher T. 2016. Preparation and fluorescent analysis of plant metaphase chromosomes. Chapter 7. In: Plant Cell Division: Methods and Protocols, ed Caillaud M-C; Methods in Molecular Biology 1370: 87-103. doi: 10.1007/978-1-4939-3142-2_7. Humana Press, Springer, New York. Local Copy: Chromosome Preparation. Trude Schwarzacher. http://www.molcyt.com Good preparations are essential for informative analysis of both somatic and…
The molecular cytogenetic characterization of pistachio (Pistacia vera) suggests the arrest of recombination in the largest heteropycnotic pair HC1
TS. Sola-Campoy PJ, Robles F, Schwarzacher T, Ruiz Rejón C, de la Herrán R, Navajas-Pérez R (2015) The molecular cytogenetic characterization of Pistachio (Pistacia vera L.) suggests the arrest of recombination in the largest heteropycnotic pair HC1. PLoS ONE 10(12): e0143861. doi:10.1371/journal.pone.0143861. Local copy here. This paper represents the first molecular cytogenetic characterization of the…
Intergeneric hybridisation between Berula erecta and Helosciadium nodiflorum (Apiaceae)
TS. Desjardins SD, Leslie AC, Stace CA, Schwarzacher T, Bailey JP. 2015. Intergeneric hybridisation between Berula erecta and Helosciadium nodiflorum (Apiaceae). Taxon 64: 784-794. DOI: 10.12705/644.9 Publisher link££ Author version Intergeneric hybridisation A hybrid between Berula erecta and Helosciadium nodiflorum is reported from Chippenham Fen National Nature Reserve (NNR), Cambridgeshire, England. Parentage was investigated using chromosome counts, a maternally-inherited chloroplast marker…
Genetic and physical maps of the Primula vulgaris S locus and localization by chromosome in situ hybridization
314. Li J, Webster MA, Wright J, Cocker JM, Smith MC, Badakshi F, Heslop‐Harrison P, Gilmartin PM. 2015. Integration of genetic and physical maps of the Primula vulgaris S locus and localization by chromosome in situ hybridization. New Phytologist http://onlinelibrary.wiley.com/doi/10.1111/nph.13373/full. dx.doi.org/10.1111/nph.13373 Heteromorphic flower development in Primula is controlled by the S locus. The S locus genes, which…
Molecular cytogenetics research group October 2014
At our lab. meeting a couple of weeks ago, we were happy to welcome two new PhD students to the group. Here we all are in the lobby of our Adrian Building. We are from six countries, and work on a diverse range of molecular cytogenetics projects. From left to right, left of DNA molecule:…
The origin of an old, resource-efficient crop, Broomcorn millet or Panicum miliaceum
307. Hunt HV, Badakshi F, Romanova O, Howe CJ, Jones M, Heslop-Harrison JS. 2014. Reticulate evolution in Panicum (Poaceae): the origin of tetraploid broomcorn millet, P. miliaceum. Journal of Experimental Botany 65 (12), 3165-3175. DOI:10.1093/jxb/eru161 . (Link to local copy J. Exp. Bot.-2014-Hunt-3165-75) Panicum miliaceum (broomcorn millet) is a tetraploid cereal which was among the first domesticated crops, but is now a minor…
In situ hybridization: a course on FISH and ChIp
In situ hybridization – a course on FISH and ChIp In situ hybridization, now largely with fluorescent detection so ‘fluorescent in situ hybridization’ or FISH, is a key method for localizing DNA sequences along chromosomes and occassionally within interphase nuclei. RNA in situ hybridization (not discussed here) is used for locating gene transcripts within nuclei….
Alien chromosome introgression transferring characters into bread wheat
In situ hybridization is now widely used to identify the nature and size of chromosome, chromosome arm and chromosome segment introgressions from alien or wild species in plant breeding lines. The molecular cytogenetics research group has several projects investigating introgression of chromosomes into wheat from rye, Thinopyrum (2x and 6x), Aegilops and other species or…
Plant nuclear genome composition
299. Heslop-Harrison, JS (Pat); and Schmidt, T (August 2012) Plant Nuclear Genome Composition. In: eLS 2012, John Wiley & Sons Ltd: Chichester http://www.els.net/ Author-prepared preprint linked here. Subscription DOI: http://onlinelibrary.wiley.com/doi/10.1002/9780470015902.a0002014.pub2/abstract (but this link seems not to work: http://dx.doi.org/10.1002/9780470015902.a0002014.pub2 Nov 2012). Intranet: http://www.le.ac.uk/biology/phh4/HHpubs/ELS_PlantGenomes_SchmidtHH.pdf due Nov 2012). The plant nuclear genome consists of DNA divided among the chromosomes within the cell nucleus. Plant…
Trude Schwarzacher PI
Dr Trude Schwarzacher is a co-project leader of the Molecular Cytogenetics research group in the University of Leicester. Current research interests include: Wheat and related species I am studying introgression of alien chromosomes and introgression of alien chromosomes from wild species into bread wheat. In wheat and related species, we have found differences in cytosine…
Molecular cytogenetics, genome evolution and agricultural genomics 