Tag Archives: in situ hybridization

Complete mitogenomes from Kurdistani sheep – abundant centromeric nuclear copies representing diverse ancestors

341. Mustafa SI, Schwarzacher T, and Heslop-Harrison JS. 2018. Complete mitogenomes from Kurdistani sheep: abundant centromeric nuclear copies representing diverse ancestors.  Mitochondrial DNA Part A https://doi.org/10.1080/24701394.2018.1431226 (publisher – see free publisher link below if you can’t access) AND Mustafa et al_2018 AuthorVersion … Continue reading

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CENH3 morphogenesis reveals dynamic centromere associations during synaptonemal complex formation and the progression through male meiosis in hexaploid wheat

330. Sepsi A, Higgins JD, Heslop-Harrison JS, Schwarzacher T. 2017. CENH3 morphogenesis reveals dynamic centromere associations during synaptonemal complex formation and the progression through male meiosis in hexaploid wheat. Plant Journal 89: 235-249. http://dx.doi.org/10.1111/tpj.13379 and Author Version: Sepsi et al. 2016. … Continue reading

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Introgression of chromosome segments from multiple alien species in wheat breeding lines with wheat streak mosaic virus resistance

324. Ali N, Heslop-Harrison JS, Ahmad H, Graybosch RA, Hein GL, Schwarzacher T. 2016. Introgression of chromosome segments from multiple alien species in wheat breeding lines with wheat streak mosaic virus resistance. Heredity (2016) 117, 114–123; published online 1 June … Continue reading

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Analysis of Petunia vein clearing virus (PVCV) sequences, retroelements and tandem repeats in Petunia axillaris N and P. inflata S6

325a. Schwarzacher T, Heslop-Harrison JS, Richert-Pöggeler KR. 2016. Analysis of Petunia vein clearing virus (PVCV) sequences, retroelements and tandem repeats in Petunia axillaris N and P. inflata S6. Supplementary manuscript 2. Nature Plants 2: article number 16074. Link to manuscript: Petunia_PVCV_Repeats_SchwarzacherEtAl2016 Within … Continue reading

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Preparation and fluorescent analysis of plant metaphase chromosomes

TS. Schwarzacher T. 2016. Preparation and fluorescent analysis of plant metaphase chromosomes. Chapter 7. In: Plant Cell Division: Methods and Protocols, ed Caillaud M-C; Methods in Molecular Biology 1370: 87-103. doi: 10.1007/978-1-4939-3142-2_7. Humana Press, Springer, New York. Local Copy: Chromosome … Continue reading

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The molecular cytogenetic characterization of pistachio (Pistacia vera) suggests the arrest of recombination in the largest heteropycnotic pair HC1

TS. Sola-Campoy PJ, Robles F, Schwarzacher T, Ruiz Rejón C, de la Herrán R, Navajas-Pérez R (2015) The molecular cytogenetic characterization of Pistachio (Pistacia vera L.) suggests the arrest of recombination in the largest heteropycnotic pair HC1. PLoS ONE 10(12): … Continue reading

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Intergeneric hybridisation between Berula erecta and Helosciadium nodiflorum (Apiaceae)

TS. Desjardins SD, Leslie AC, Stace CA, Schwarzacher T,  Bailey JP. 2015. Intergeneric hybridisation between Berula erecta and Helosciadium nodiflorum (Apiaceae). Taxon 64: 784-794. DOI: 10.12705/644.9 Publisher link££ Author version Intergeneric hybridisation A hybrid between Berula erecta and Helosciadium nodiflorum is reported from Chippenham Fen National … Continue reading

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Genetic and physical maps of the Primula vulgaris S locus and localization by chromosome in situ hybridization

314. Li J, Webster MA, Wright J, Cocker JM, Smith MC, Badakshi F, Heslop‐Harrison P, Gilmartin PM. 2015. Integration of genetic and physical maps of the Primula vulgaris S locus and localization by chromosome in situ hybridization. New Phytologist http://onlinelibrary.wiley.com/doi/10.1111/nph.13373/full. dx.doi.org/10.1111/nph.13373 Heteromorphic … Continue reading

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Molecular cytogenetics research group October 2014

At our lab. meeting a couple of weeks ago, we were happy to welcome two new PhD students to the group. Here we all are in the lobby of our Adrian Building. We are from six countries, and work on … Continue reading

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The origin of an old, resource-efficient crop, Broomcorn millet or Panicum miliaceum

307. Hunt HV, Badakshi F, Romanova O, Howe CJ, Jones M, Heslop-Harrison JS. 2014. Reticulate evolution in Panicum (Poaceae): the origin of tetraploid broomcorn millet, P. miliaceum. Journal of Experimental Botany 65 (12), 3165-3175.  DOI:10.1093/jxb/eru161 . (Link to local copy J. Exp. Bot.-2014-Hunt-3165-75) Panicum miliaceum (broomcorn millet) … Continue reading

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In situ hybridization: a course on FISH and ChIp

In situ hybridization – a course on FISH and ChIp In situ hybridization, now largely with fluorescent detection so ‘fluorescent in situ hybridization’ or FISH, is a key method for localizing DNA sequences along chromosomes and occassionally within interphase nuclei. … Continue reading

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Alien chromosome introgression transferring characters into bread wheat

In situ hybridization is now widely used to identify the nature and size of chromosome, chromosome arm and chromosome segment introgressions from alien or wild species in plant breeding lines. The molecular cytogenetics research group has several projects investigating introgression … Continue reading

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Plant nuclear genome composition

299. Heslop-Harrison, JS (Pat); and Schmidt, T (August 2012) Plant Nuclear Genome Composition. In: eLS 2012, John Wiley & Sons Ltd: Chichester http://www.els.net/ Author-prepared preprint linked here. Subscription DOI:  http://onlinelibrary.wiley.com/doi/10.1002/9780470015902.a0002014.pub2/abstract (but this link seems not to work: http://dx.doi.org/10.1002/9780470015902.a0002014.pub2 Nov 2012). Intranet: http://www.le.ac.uk/biology/phh4/HHpubs/ELS_PlantGenomes_SchmidtHH.pdf due Nov 2012). The … Continue reading

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Trude Schwarzacher PI

Dr Trude Schwarzacher is a co-project leader of the Molecular Cytogenetics research group in the University of Leicester. Current research interests include: Wheat and related species I am studying introgression of alien chromosomes and introgression of alien chromosomes from wild … Continue reading

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