Sarbast Ihsan Mustafa, Trude Schwarzacher and John S. Heslop-Harrison The Nature and Chromosomal Landscape of Endogenous Retroviruses (ERVs) Integrated in the Sheep Nuclear Genome. DNA 2022, 2(1), 86-103; Free full text: https://doi.org/10.3390/dna2010007 Endogenous retroviruses (ERVs) represent genomic components of retroviral origin that are found integrated in the genomes of various species of vertebrates. These genomic…
Tag: genomes
Bovine satellite DNAs and a history of the evolution of complexity and its impact in the Bovidae family
348. Escudeiro A, Ferreira D, Mendes-da-Silva A, Heslop-Harrison JS, Adega F, Chaves R. 2019. Bovine satellite DNAs–a history of the evolution of complexity and its impact in the Bovidae family. European Zoological Journal. 2019 Jan 1 86(1):20-37. https://doi.org/10.1080/24750263.2018.1558294 (Open access) Despite the many questions regarding satellite DNA sequences and their cellular roles, the evolutionary history of…
Crop Improvement – Plant Nuclear Genomes
328. Heslop-Harrison JS. 2017. Crop Improvement: Plant Nuclear Genomes. Encyclopedia of Applied Plant Sciences 2nd Edition. In proof. Plant breeders work with large amounts of DNA sequence information including the sequences of all genes and the repetitive DNA that makes up the majority of most genomes. Knowledge of the diversity and evolution of the sequences…
Chloroplast genomes from apomictic Taraxacum – identity and variation between microspecies
Taraxacum or dandelion chloroplast sequence diversity. Salih et al. PLoS One 2017. 333. Salih RHM, Majeský L, Schwarzacher T, Gornall R, Heslop-Harrison P. 2017. Complete chloroplast genomes from apomictic Taraxacum (Asteraceae): Identity and variation between three microspecies. PLoS ONE 12(2): e0168008. doi:10.1371/journal.pone.0168008. Chloroplast DNA sequences show substantial variation between higher plant species, and less variation within…
Molecular Cytogenetics Group – an infographic of what we do
Infographic: what we do in the Molecular Cytogenetics Research Group
Preparation and fluorescent analysis of plant metaphase chromosomes
TS. Schwarzacher T. 2016. Preparation and fluorescent analysis of plant metaphase chromosomes. Chapter 7. In: Plant Cell Division: Methods and Protocols, ed Caillaud M-C; Methods in Molecular Biology 1370: 87-103. doi: 10.1007/978-1-4939-3142-2_7. Humana Press, Springer, New York. Local Copy: Chromosome Preparation. Trude Schwarzacher. http://www.molcyt.com Good preparations are essential for informative analysis of both somatic and…
Plant Virus Evolution and Pararetroviruses in Petunia
TS. Hohn T, Richert-Pöggeler KR, Staginnus C, Harper G, Schwarzacher T, Teo CH, Teycheney P-Y, Iskra-Caruana M-L, Hull R. 2008. Evolution of Integrated Plant Viruses. Chapter 4 pp 53-81. In: Plant Virus Evolution Ed Roossinck MJ. Springer: Berlin Link to Publisher homepage about the book with links to downloadable copies of the whole book or the chapter….
Evidence of convergent evolution in humans and macaques supports an adaptive role for copy number variation of the β-defensin-2 gene
Ottolini B, Hornsby MJ, Abujaber R, Macarthur JA, Badge RM, Schwarzacher T, Albertson DG, Bevins CL, Solnick JV, Hollox EJ. 2014. Evidence of convergent evolution in humans and macaques supports an adaptive role for copy number variation of the β-defensin-2 gene. Genome Biology and Evolution 6(11): 3025-3038. DOI: 10.1093/gbe/evu236 β-defensins are a family of important peptides of…
In situ hybridization: a course on FISH and ChIp
In situ hybridization – a course on FISH and ChIp In situ hybridization, now largely with fluorescent detection so ‘fluorescent in situ hybridization’ or FISH, is a key method for localizing DNA sequences along chromosomes and occassionally within interphase nuclei. RNA in situ hybridization (not discussed here) is used for locating gene transcripts within nuclei….
Repetitive DNA in tetraploid peanut and its evolution from diploid Arachis
302. Bertioli DJ, Vidigal B, Nielen S, Ratnaparkhe MB, Lee T-JH, Leal-Bertioli SCM, Kim C, Guimaraes PM, Seijo G, Schwarzacher T, Paterson AH, Heslop-Harrison P, Araujo ACG. 2013. The repetitive component of the A genome of peanut (Arachis hypogaea) and its role in remodelling intergenic sequence space since its evolutionary divergence from the B genome. Annals…
Nouf Alsayied: PhD student with Saffron Crocus at the University of Leicester and faculty member Umm Al-Qura University, Kingdom of Saudi Arabia
My research focuses on understanding the origin and diversity of the genus Crocus (Iridaceae), with particular emphasis on a triploid sterile plant, Crocus sativus (2n=3x=24) that yields Saffron, one of the most expensive spices on earth. The saffron is the stigmas of the flowers, and nearly 200,000 must be collected to give 1kg of spice,…
Genetic stability or variation in oil palm tissue culture
301. Madon M, Heslop-Harrison JS, Schwarzacher T, Hashim AT. 2012. Analysis of oil palm calli and regenerants using flow and image cytometry and 18S-25S ribosomal DNA fluorescence in situ hybridisation (FISH). Journal of Oil Palm Research 24 (April): 1318-1329 Open Access Journal site. local copy Flow (FCM) and image cytometry (ICM) and 18S-25S ribosomal DNA (rDNA) fluorescence in situ hybridisation…
Plant nuclear genome composition
299. Heslop-Harrison, JS (Pat); and Schmidt, T (August 2012) Plant Nuclear Genome Composition. In: eLS 2012, John Wiley & Sons Ltd: Chichester http://www.els.net/ Author-prepared preprint linked here. Subscription DOI: http://onlinelibrary.wiley.com/doi/10.1002/9780470015902.a0002014.pub2/abstract (but this link seems not to work: http://dx.doi.org/10.1002/9780470015902.a0002014.pub2 Nov 2012). Intranet: http://www.le.ac.uk/biology/phh4/HHpubs/ELS_PlantGenomes_SchmidtHH.pdf due Nov 2012). The plant nuclear genome consists of DNA divided among the chromosomes within the cell nucleus. Plant…
Welcome to the MolCyt Molecular Cytogenetics blog
Welcome to the Molecular Cytogenetics http://www.MolCyt.org blog site. This blog feeds to the http://www.molcyt.com website and provides updates. Blog posts are in categories of Publications, Research, People/Collaborators, Conferences, Teaching, Social Media, Editing, Methods, and News/Press.
Molecular cytogenetics, genome evolution and agricultural genomics 