In situ hybridization – a course on FISH and ChIp In situ hybridization, now largely with fluorescent detection so ‘fluorescent in situ hybridization’ or FISH, is a key method for localizing DNA sequences along chromosomes and occassionally within interphase nuclei. RNA in situ hybridization (not discussed here) is used for locating gene transcripts within nuclei….
Month: December 2013
Spectroscopy and DNA concentration measurement
DNA concentration measurement is fundamental to most molecular biology methods. What is the basis of DNA concentration measurement? I’m always happy to find an article which has detail at the level you need to know! In a free magazine, VWR BioMarkers, there is a very useful article from GE Life Sciences. http://media.vwr.com/interactive/publications/VWR_bioMarke_2013/Magazine/files/assets/basic-html/index.html#7 A local copy…
From vegan to meat: human diet and trophic levels
Where are humans in the food chain? Bonhommeau et al. address this question in PNAS, apparently for the first time, in detail across all countries and over the period from 1961 that FAO statistics on food consumption and production are available (FAOstat.FAO.org). If we ate only the primary producers – plants – we would have a…
Molecular cytogenetics, genome evolution and agricultural genomics 