Preparation and fluorescent analysis of plant metaphase chromosomes

TS. Schwarzacher T. 2016. Preparation and fluorescent analysis of plant metaphase chromosomes. Chapter 7. In: Plant Cell Division: Methods and Protocols, ed Caillaud M-C; Methods in Molecular Biology 1370: 87-103. doi: 10.1007/978-1-4939-3142-2_7. Humana Press, Springer, New York. Local Copy: Chromosome Preparation. Trude Schwarzacher. http://www.molcyt.com Good preparations are essential for informative analysis of both somatic and…

Chromosomal distribution and evolution of retrotransposons in diploid and polyploid Brachiaria forage grasses

319. Santos FC, Guyot R, Valle CB do, Chiari L, Techio VH, Heslop-Harrison P, Vanzela ALL. 2015. Chromosomal distribution and evolution of abundant retrotransposons in plants: gypsy elements in diploid and polyploid Brachiaria forage grasses. Chromosome Research 23(3): 571-582. DOI: 10.1007/s10577-015-9492-6 Text Brachiaria_Fabiola_Santos_etal AuthorVersion. Abstract: Like other eukaryotes, the nuclear genome of plants consists of DNA with…

Wheat-Thinopyrum bessarabicum recombinant lines with intercalary translocations

318. Patokar C, Sepsi A, Schwarzacher T, Kishii M, Heslop-Harrison JS. 2016. Molecular cytogenetic characterization of novel wheat-Thinopyrum bessarabicum recombinant lines carrying intercalary translocations. Chromosoma 125: 163–172. 10.1007/s00412-015-0537-6 Publisher website. Version from Wheat-Thinopyrum introgression and chromosomal recombinants. Thinopyrum bessarabicum (2n=2x=14, JJ or EbEb) is a valuable source of genes for bread wheat (2n = 6x=42) improvement because…

Alien chromosome introgression transferring characters into bread wheat

In situ hybridization is now widely used to identify the nature and size of chromosome, chromosome arm and chromosome segment introgressions from alien or wild species in plant breeding lines. The molecular cytogenetics research group has several projects investigating introgression of chromosomes into wheat from rye, Thinopyrum (2x and 6x), Aegilops and other species or…