Plant nuclear genome composition

Aspects of the Plant Nuclear Genome (ELS article)

Aspects of the Plant Nuclear Genome (ELS article)

299. Heslop-Harrison, JS (Pat); and Schmidt, T (August 2012) Plant Nuclear Genome Composition. In: eLS 2012, John Wiley & Sons Ltd: Chichester http://www.els.net/

Author-prepared preprint linked here. Subscription DOI:  http://onlinelibrary.wiley.com/doi/10.1002/9780470015902.a0002014.pub2/abstract

(but this link seems not to work: http://dx.doi.org/10.1002/9780470015902.a0002014.pub2 Nov 2012). Intranet: http://www.le.ac.uk/biology/phh4/HHpubs/ELS_PlantGenomes_SchmidtHH.pdf due Nov 2012).

The plant nuclear genome consists of DNA divided among the chromosomes within the cell nucleus. Plant genomes contain coding and regulatory sequences for genes and repetitive DNA. They are evolutionarily dynamic and analysis provides insights into the evolution of genes and genomes, supporting studies of species phylogeny and plant breeding. The amount of DNA present in plant genomes, nearly constant within one species, varies over some 2300 times between species; the majority of the difference is accounted for genome duplication, or by various classes of repetitive DNA which may be dispersed widely along chromosomes or located in arrays at a small number of loci. Bioinformatics and genome sequence analysis show conservation of many gene sequences across all plants. Repetitive DNA motifs may be conserved over large taxonomic groupings or evolve rapidly. Repetitive DNA plays an important role as a structural component of plant chromosomes (e.g. telomeres and centromeres) and affects gene regulation.

Keywords: tandemly repeated DNA; transposable elements; in situ hybridization; chromosomes; genomes; nucleus; retrotransposons; repetitive DNA; junk DNA; satellites; microsatellites; heterochromatin;

Key Concepts:

  • Plant nuclear genome sizes, constant in a species, vary in size from 60,000,000 base pairs of DNA (written as 60 Mbp) to 150,000 Mbp, a range of 2300 times.
  • The DNA of plant nuclear genomes is wrapped around the histone proteins to form nucleosomes, and the resulting chromatin is organized into linear chromosomes with characteristic numbers, sizes and morphology in each species.
  • Genomes contain coding sequences, typically for 25,000 to 40,000 genes, and regulatory sequences, as shown by genome sequencing and bioinformatics.
  • Size variation comes from polyploidization, genome or chromosome duplication, and particularly amplification of DNA motifs to give repetitive DNA.
  • Repetitive DNA consists of sequences that amplify via RNA intermediates (retrotransposons), through copying and reinsertion of DNA (DNA transposable elements),  or other mechanisms (satellite and microsatellite DNA)
  • Some repetitive DNA encodes genes, in particular the 45S and the 5S ribosomal RNA genes (rDNA); other repetitive DNA has structural roles at the telomeres or chromosomal ends and the centromeres where chromosomes attach to microtubules during segregation. In most species, much of the repetitive DNA has no known function.
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About Pat Heslop-Harrison

Professor of Molecular Cytogenetics and Cell Biology, University of Leicester Chief Editor, Annals of Botany. Research: genome evolution, breeding and biodiversity in agricultural species; the impact of agriculture; evalutation of research and advanced training.
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One Response to Plant nuclear genome composition

  1. Pingback: Repetitive DNA in the edible button mushroom | Molecular cytogenetics and genome evolution

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