Genetic linkage mapping in an F2 perennial ryegrass population using DArT markers

Lolium trials for genetic mapping

Lolium trials for genetic mapping

292. Tomaszewski C, Byrne SL, Foito A, Kildea A, Kopecký D, Doležel J, Heslop-Harrison JS, Stewart D, Barth S. 2012. Genetic linkage mapping in an F2 perennial ryegrass population using DArT markers. Plant Breeding 131, 345—349.

Publisher: DOI: 10.1111/j.1439-0523.2011.01944.x.

Intranet only link (needs login).

Perennial ryegrass is the principal forage grass species used in temperate agriculture. In recent years, significant efforts have been made to develop molecular marker strategies to allow cost-effective characterization of a large number of loci simultaneously. One such strategy involves using DArT markers, and a DArT array has recently been developed for the Lolium-Festuca complex. In this study, we report the first use of the DArTFest array to generate a genetic linkage map based on 326 markers in a Lolium perenne F2 population, consisting of 325 genotypes. For proof of concept, the map was used to identify QTL associated with differences in crown
rust susceptibility, caused by the fungal biotroph, Puccinia coronata.

Lolium perenne — perennial ryegrass — genetic
map — crown rust — Puccinia coronata — DArT

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About Pat Heslop-Harrison

Professor of Molecular Cytogenetics and Cell Biology, University of Leicester Chief Editor, Annals of Botany. Research: genome evolution, breeding and biodiversity in agricultural species; the impact of agriculture; evalutation of research and advanced training.
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