292. Tomaszewski C, Byrne SL, Foito A, Kildea A, Kopecký D, Doležel J, Heslop-Harrison JS, Stewart D, Barth S. 2012. Genetic linkage mapping in an F2 perennial ryegrass population using DArT markers. Plant Breeding 131, 345—349.
Publisher: DOI: 10.1111/j.1439-0523.2011.01944.x.
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Perennial ryegrass is the principal forage grass species used in temperate agriculture. In recent years, signiﬁcant eﬀorts have been made to develop molecular marker strategies to allow cost-eﬀective characterization of a large number of loci simultaneously. One such strategy involves using DArT markers, and a DArT array has recently been developed for the Lolium-Festuca complex. In this study, we report the ﬁrst use of the DArTFest array to generate a genetic linkage map based on 326 markers in a Lolium perenne F2 population, consisting of 325 genotypes. For proof of concept, the map was used to identify QTL associated with diﬀerences in crown
rust susceptibility, caused by the fungal biotroph, Puccinia coronata.
Lolium perenne — perennial ryegrass — genetic
map — crown rust — Puccinia coronata — DArT