Molecular characterization and diversity of a novel nonautonomous Mutator-like transposon family in Brassica

Brassica Mutatot-like MULE transposons. Nouroz et al. 2015

Brassica Mutatot-like MULE transposons. Nouroz et al. 2015

320. Nouroz F, Noreen S, Heslop-Harrison JS. 2015. Molecular characterization and diversity of a novel nonautonomous Mutator-like transposon family in Brassica. Pakistani Journal of Botany 47(4): 1367-1375. Publisher abstract Publisher free full text. Local copy BrassicaMutator_Nouroz_PakistaniJBot2015

Transposable elements (TEs) are capable of mobilizing from one genomic location to other, with changes in their copy numbers. Mutator-like elements (MULEs) are DNA transposons characterized by 9 bp target site duplications (TSDs), with high variability in sequence and length, and include non-conserved terminal inverted repeats (TIRs). We identified and characterized a family of Mutator-like elements designated as Shahroz. The structural and molecular analyses revealed that family had a small number of mostly defective non-autonomous MULEs and has shown limited activity in the evolutionary history of the Brassica A-genome. The Shahroz elements range in size from 2734 to 3160 bp including 76 bp imperfect TIRs and 9 bp variable TSDs. The individual copies have shown high homology (52–99%) in their entire lengths. The study revealed that the elements are less in numbers but active in Brassica rapa genomes and PCR amplification revealed their specificity and amplification in A-genome containing diploid and polyploids Brassica. The phylogenetic analysis of Brassica MULEs with other plant Mutator elements revealed that no correlation exists between Brassica MULEs and other elements suggesting a separate line of evolution. Analyzing the regions flanking the insertions revealed that the insertions have showed a preference for AT rich regions. The detailed study of these insertions revealed that although less in number and small sizes, they have played a role in Brassica genome evolution by their mobilization.

Key words: Transposable elements, Brassica, Mutator, Diversity, Shahroz, Phylogenetic analysis

About Pat Heslop-Harrison

Professor of Molecular Cytogenetics and Cell Biology, University of Leicester. Group Leader, South China Botanical Garden, Chinese Academy of Sciences. Chief Editor, Annals of Botany. Research: genome evolution, breeding and biodiversity in agricultural species; the impact of agriculture; evalutation of research and advanced training.
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