Analysis of Petunia vein clearing virus (PVCV) sequences, retroelements and tandem repeats in Petunia axillaris N and P. inflata S6

325a. Schwarzacher T, Heslop-Harrison JS, Richert-Pöggeler KR. 2016. Analysis of Petunia vein clearing virus (PVCV) sequences, retroelements and tandem repeats in Petunia axillaris N and P. inflata S6. Supplementary manuscript 2. Nature Plants 2: article number 16074. Link to manuscript: Petunia_PVCV_Repeats_SchwarzacherEtAl2016 Within the genome sequence assemblies of P. axillaris (PaxiN) and P. inflata (PinfS6) and unassembled reads, we…

Regulation should follow robust scientific assessments not opinions

University of Leicester scientist in battle to ‘stem onslaught of pseudoscience’ Geneticist at European Commission alarmed at impact of ‘dogma’ in regulation and the negative impact on the environment and European industry A University of Leicester scientist has joined leading scientists from across Europe in raising an alarm over the ‘pseudoscience’ concerning regulation of compounds used…

Saffron crocus, cooking and Iran on the radio

The spice Saffron is valued worldwide for its flavor, aroma and color. I’ve just broadcast an interview with Robin Young produced by Jill Ryan for NPR’s Here and Now program which let me tell you something about my enjoyment of saffron both as a consumer and scientist. Saffron is unusual in that it is equally…

Preparation and fluorescent analysis of plant metaphase chromosomes

TS. Schwarzacher T. 2016. Preparation and fluorescent analysis of plant metaphase chromosomes. Chapter 7. In: Plant Cell Division: Methods and Protocols, ed Caillaud M-C; Methods in Molecular Biology 1370: 87-103. doi: 10.1007/978-1-4939-3142-2_7. Humana Press, Springer, New York. Local Copy: Chromosome Preparation. Trude Schwarzacher. http://www.molcyt.com Good preparations are essential for informative analysis of both somatic and…

The molecular cytogenetic characterization of pistachio (Pistacia vera) suggests the arrest of recombination in the largest heteropycnotic pair HC1

TS. Sola-Campoy PJ, Robles F, Schwarzacher T, Ruiz Rejón C, de la Herrán R, Navajas-Pérez R (2015) The molecular cytogenetic characterization of Pistachio (Pistacia vera L.) suggests the arrest of recombination in the largest heteropycnotic pair HC1. PLoS ONE 10(12): e0143861. doi:10.1371/journal.pone.0143861. Local copy here. This paper represents the first molecular cytogenetic characterization of the…

Repetitive DNA in eukaryotic genomes

322. Biscotti MA, Olmo E, Heslop-Harrison JS. 2015. Repetitive DNA in eukaryotic genomes. Chromosome Research 23(3): 415-420. DOI: 10.1007/s10577-015-9499-z Biscotti Repetitive DNA Author Version Link to Repetitive DNA summary Diagram Molcyt.com DNA Repetitive DNA — sequence motifs repeated hundreds or thousands of times in the genome— makes up the major proportion of all the nuclear DNA in…

Chromosomal distribution and evolution of retrotransposons in diploid and polyploid Brachiaria forage grasses

319. Santos FC, Guyot R, Valle CB do, Chiari L, Techio VH, Heslop-Harrison P, Vanzela ALL. 2015. Chromosomal distribution and evolution of abundant retrotransposons in plants: gypsy elements in diploid and polyploid Brachiaria forage grasses. Chromosome Research 23(3): 571-582. DOI: 10.1007/s10577-015-9492-6 Text Brachiaria_Fabiola_Santos_etal AuthorVersion. Abstract: Like other eukaryotes, the nuclear genome of plants consists of DNA with…

dsRNA silencing of an R2R3-MYB transcription factor affects flower cell shape in a Dendrobium hybrid

TS. Lau S-E, Schwarzacher T, Othman RY, Harikrishna JA. 2015. dsRNA silencing of an R2R3-MYB transcription factor affects flower cell shape in a Dendrobium hybrid. BMC Plant Biology 15, no. 1: 194. http://www.biomedcentral.com/1471-2229/15/194 DOI 10.1186/s12870-015-0577-3 Local copy Lau et al Dendrobium dsRNA silencing BMCPlant Biology 2015 Background: The R2R3-MYB genes regulate pigmentation and morphogenesis of flowers, including flower and cell shape,…

Wheat-Thinopyrum bessarabicum recombinant lines with intercalary translocations

318. Patokar C, Sepsi A, Schwarzacher T, Kishii M, Heslop-Harrison JS. 2016. Molecular cytogenetic characterization of novel wheat-Thinopyrum bessarabicum recombinant lines carrying intercalary translocations. Chromosoma 125: 163–172. 10.1007/s00412-015-0537-6 Publisher website. Version from Wheat-Thinopyrum introgression and chromosomal recombinants. Thinopyrum bessarabicum (2n=2x=14, JJ or EbEb) is a valuable source of genes for bread wheat (2n = 6x=42) improvement because…

Identification and evolutionary genomics of novel LTR retrotransposons in Brassica

315. Nouroz F, Noreen S, Heslop-Harrison JS. 2015. Identification and evolutionary genomics of Novel LTR retrotransposons in Brassica. Turkish Journal of Biology 39: 740-757 DOI: 10.3906/biy-1501-77 Link to manuscript PDF download at publisher site Retrotransposons (REs) are the most abundant and diverse elements identified from eukaryotic genomes. Using computational and molecular methods, 262 intact LTR retrotransposons…

Evolutionary genomics of miniature inverted-repeat transposable elements (MITEs) in Brassica

317. Nouroz F, Noreen S, Heslop-Harrison JS. 2015. Evolutionary genomics of miniature inverted-repeat transposable elements (MITEs) in Brassica. Molecular Genetics and Genomics (MGG) 290 (6): 2297–2312. DOI: 10.1007/s00438-015-1076-9 Brassica MITEs Author Version MGG download Miniature inverted-repeat transposable elements (MITEs) are truncated derivatives of autonomous DNA transposons, and are dispersed abundantly in most eukaryotic genomes. We aimed to characterize various MITEs families…

Plant Virus Evolution and Pararetroviruses in Petunia

TS. Hohn T, Richert-Pöggeler KR, Staginnus C, Harper G, Schwarzacher T, Teo CH, Teycheney P-Y, Iskra-Caruana M-L, Hull R. 2008. Evolution of Integrated Plant Viruses. Chapter 4 pp 53-81. In: Plant Virus Evolution Ed Roossinck MJ. Springer: Berlin Link to Publisher homepage about the book with links to downloadable copies of the whole book or the chapter….

Diversity and characters in Ethiopian linseed Linum #PAGXXIII Negash Worku

Many people have talked about Orphan crops – those where there has been little genetic or other research – and the characterization, evaluation and exploitation of germplasm at the #PAGXXIII Plant and Animal Genome Conference this week. Today, I am talking about work by Negash Worku on the Diversity and Characters in Ethiopian Linseed Accessions….